The 5S gene internal control region is composed of three distinct sequence elements, organized as two functional domains with variable spacing
Identifieur interne : 004C82 ( Main/Exploration ); précédent : 004C81; suivant : 004C83The 5S gene internal control region is composed of three distinct sequence elements, organized as two functional domains with variable spacing
Auteurs : Tomas Pieler [Allemagne] ; Jörg Hamm [Allemagne] ; R. G. Roeder [États-Unis]Source :
- Cell [ 0092-8674 ] ; 1987.
English descriptors
- Teeft :
- Assay, Biol, Bogenhagen, Chem, Ciliberto, Complex formation, Control region, Deletion, Deletion mutants, Distinct sequence elements, Dnaase, Eukaryotic, Eukaryotic trna genes, Factor binding, Gene, Gene promoter, Gene transcription, Individual steps, Insertion, Intermediate element, Internal control region, Laevis, Lassar, Linker, Lower affinity, Major promoter domains, Mutagenesis, Mutant, Mutation, Nucleotide, Pieler, Point mutants, Point mutations, Polymerase, Preincubation, Promoter, Promoter domain, Promoter element, Promoter elements, Protein binding, Relative affinity, Roeder, Room temperature, Sakonju, Second gene, Sequence element, Spacer, Spacer mutants, Stable initiation, Structural information, Tdna, Tfiiia, Tfiiia binding, Tfiiib, Tfiiic, Tfiiic binding, Transcription, Transcription activity, Transcription factor, Transcription factors, Transcription initiation, Trna, Trna genes, Trna signal, Trna transcription, Xenopus, Xenopus laevis.
Abstract
Abstract: Systematic oligonucleotide-directed mutagenesis within the internal control region of the Xenopus laevis somatic 5S RNA gene identifies three distinct sequence elements that regulate transcription activity: box A, containing the common, conserved class III promoter domain, and two 5S-gene-specific segments, termed intermediate element and box C. Analysis of the individual steps in the formation of the stable initiation complex reveals that the two 5S-gene-specific elements are the main determinants for the stable binding of TFIIIA. In contrast, TFIIIC binding appears to be dependent on interactions with TFIIIA and on direct DNA interactions in box A as well as probably in box C. Alterations of the spacing between the two major promoter domains of from −3 to +10 nucleotides are tolerated, although they reduce transcription activity and were found to prevent the formation of a stable initiation complex.
Url:
DOI: 10.1016/0092-8674(87)90359-X
Affiliations:
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Roeder</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">État de New York</region></placeName><wicri:cityArea>Laboratory of Biochemistry and Molecular Biology The Rockefeller University New York</wicri:cityArea></affiliation></author></analytic><monogr></monogr><series><title level="j">Cell</title><title level="j" type="abbrev">CELL</title><idno type="ISSN">0092-8674</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1987">1987</date><biblScope unit="volume">48</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="91">91</biblScope><biblScope unit="page" to="100">100</biblScope></imprint><idno type="ISSN">0092-8674</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0092-8674</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Assay</term><term>Biol</term><term>Bogenhagen</term><term>Chem</term><term>Ciliberto</term><term>Complex formation</term><term>Control region</term><term>Deletion</term><term>Deletion mutants</term><term>Distinct sequence elements</term><term>Dnaase</term><term>Eukaryotic</term><term>Eukaryotic trna genes</term><term>Factor binding</term><term>Gene</term><term>Gene promoter</term><term>Gene transcription</term><term>Individual steps</term><term>Insertion</term><term>Intermediate element</term><term>Internal control region</term><term>Laevis</term><term>Lassar</term><term>Linker</term><term>Lower affinity</term><term>Major promoter domains</term><term>Mutagenesis</term><term>Mutant</term><term>Mutation</term><term>Nucleotide</term><term>Pieler</term><term>Point mutants</term><term>Point mutations</term><term>Polymerase</term><term>Preincubation</term><term>Promoter</term><term>Promoter domain</term><term>Promoter element</term><term>Promoter elements</term><term>Protein binding</term><term>Relative affinity</term><term>Roeder</term><term>Room temperature</term><term>Sakonju</term><term>Second gene</term><term>Sequence element</term><term>Spacer</term><term>Spacer mutants</term><term>Stable initiation</term><term>Structural information</term><term>Tdna</term><term>Tfiiia</term><term>Tfiiia binding</term><term>Tfiiib</term><term>Tfiiic</term><term>Tfiiic binding</term><term>Transcription</term><term>Transcription activity</term><term>Transcription factor</term><term>Transcription factors</term><term>Transcription initiation</term><term>Trna</term><term>Trna genes</term><term>Trna signal</term><term>Trna transcription</term><term>Xenopus</term><term>Xenopus laevis</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Systematic oligonucleotide-directed mutagenesis within the internal control region of the Xenopus laevis somatic 5S RNA gene identifies three distinct sequence elements that regulate transcription activity: box A, containing the common, conserved class III promoter domain, and two 5S-gene-specific segments, termed intermediate element and box C. Analysis of the individual steps in the formation of the stable initiation complex reveals that the two 5S-gene-specific elements are the main determinants for the stable binding of TFIIIA. In contrast, TFIIIC binding appears to be dependent on interactions with TFIIIA and on direct DNA interactions in box A as well as probably in box C. Alterations of the spacing between the two major promoter domains of from −3 to +10 nucleotides are tolerated, although they reduce transcription activity and were found to prevent the formation of a stable initiation complex.</div></front></TEI><affiliations><list><country><li>Allemagne</li><li>États-Unis</li></country><region><li>Bade-Wurtemberg</li><li>District de Karlsruhe</li><li>État de New York</li></region><settlement><li>Heidelberg</li></settlement></list><tree><country name="Allemagne"><noRegion><name sortKey="Pieler, Tomas" sort="Pieler, Tomas" uniqKey="Pieler T" first="Tomas" last="Pieler">Tomas Pieler</name></noRegion><name sortKey="Hamm, Jorg" sort="Hamm, Jorg" uniqKey="Hamm J" first="Jörg" last="Hamm">Jörg Hamm</name><name sortKey="Hamm, Jorg" sort="Hamm, Jorg" uniqKey="Hamm J" first="Jörg" last="Hamm">Jörg Hamm</name></country><country name="États-Unis"><region name="État de New York"><name sortKey="Roeder, R G" sort="Roeder, R G" uniqKey="Roeder R" first="R. G." last="Roeder">R. G. Roeder</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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